In vitro and in vivo gene delivery using a lentiviral vector
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چکیده
منابع مشابه
Expression of Recombinant Alpha-1 Antitrypsin in CHO and COS-7 Cell Lines Using Lentiviral Vector
In this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (AAT) with correct post-translational modifications, a protein production system based on the transduction of CHO and COS-7 cells using lentiviral vectors was developed. Human AAT cDNA was cloned into a replication-defective lentiviral vector. The transgene AAT-Jred chimer was transferr...
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Objective(s): Neural stem/progenitor cells (NS/PCs) hold a great potential for delivery of therapeutic agents into the injured regions of the brain. Efficient gene delivery using NS/PCs may correct a genetic defect, produce therapeutic proteins or neurotransmitters, and modulate enzyme activation. Here, we investigated the efficiency of a recombinant lentivirus vector ...
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Lentiviral vectors are promising gene delivery tools capable of transducing a variety of dividing and non-dividing cells, including pluripotent stem cells which are refractory for transduction by murine retroviruses. Although there is a growing debate on the safety of lentiviral vectors for gene transfer, in particular for those derived from human immunodeficiency viruses, type one (HIV-1) and ...
متن کاملالقای سلولهای دندریتیک تولروژن موشی با تنظیم کاهشی ملکول کمک تحریکی CD40 با استفاده از وکتور لنتی ویروس
Induction of Tolerogenic Murine Dendritic Cells by Downregulating the Co-stimulatory Molecule of CD40 Using Lentivirus Vector Mahmoodzadeh A1, Pourfatollah AA1, Karimi MH2, Moazzeni SM1 1Dept. of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran 2Transplantation Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran. Correspond Aut...
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